Laboratory Protocols

Download Lab protocols used in the AFLP analysis and for preparation of samples for automated sequencing.

Lab Sheets

      Extract DNA          Amplify DNA by PCR   Restrict PCR-Products

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Prepare Agarose-Gel  Run Electrophoresis     Use Micropipettors

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1 Prepare biological sample, e.g. cheek cells

2 Extract mitochondrial DNA

3 Perform PCR for clade specific region of most abundant haplotype

4 Prepare agarosegel

5 Test for successful PCR

6 Perform REnzyme action on PCR products

7 Evaluate complete REnzyme digest and analyse digest fragments


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